ND: |
ME06691859 |
PMID: |
6691859 |
LR: |
20061115 |
CED: |
19840220 |
DCO: |
19840220 |
Autoren: |
Ryu J; Treadwell BV; Mankin HJ |
Titel: |
Biochemical and metabolic abnormalities in normal and osteoarthritic human articular cartilage. |
Quelle: |
Arthritis and rheumatism; VOL: 27 (1); p. 49-57 /198401/ |
PM: |
Print |
SU: |
AIM IM |
Sprache: |
English |
CY: |
UNITED STATES |
JID: |
0370605 |
ISSN: |
0004-3591 |
CO: |
ARHEAW |
DT: |
Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
NG: |
AM16265 AM NIADDK |
Schlagwörter |
CT: |
CARBON RADIOISOTOPES/diagnostic use; CARTILAGE, ARTICULAR/*metabolism; FEMALE; HUMANS; HYALURONIC ACID/biosynthesis; MALE; OSTEOARTHRITIS/*metabolism; PROTEOGLYCANS/biosynthesis; SULFUR RADIOISOTOPES/diagnostic use; TRITIUM/diagnostic use |
CTG: |
KOHLENSTOFFRADIOISOTOPE/diagnostische Verwendung; KNORPEL, GELENK-/*Stoffwechsel; WEIBLICH; MENSCH; HYALURONSÄURE/Biosynthese; MÄNNLICH; OSTEOARTHROSE/*Stoffwechsel; PROTEOGLYCANE/Biosynthese; SCHWEFELRADIOISOTOPE/diagnostische Verwendung; TRITIUM/diagnostische Verwendung |
TE: |
Carbon Radioisotopes; Proteoglycans; Sulfur Radioisotopes; Tritium/10028-17-8; Hyaluronic Acid/9004-61-9 |
CR: |
10028-17-8; 9004-61-9 |
AB: |
Incorporation of radioactive precursors into macromolecules was studied with human normal and osteoarthritic articular cartilage organ culture. Analysis of the salt extracted matrix components separated by cesium chloride buoyant density gradient centrifugation showed an increase in the specific activities of all gradient fractions prepared from the osteoarthritic cartilage. Further analysis of these fractions showed the osteoarthritic cartilage contained 5 times as much sulfate incorporated into proteoglycans, and an even greater amount of 3H-glucosamine incorporated into material sedimenting to the middle of the gradient. Greater than half of this radioactive middle fraction appears to be hyaluronate, as judged by the position it elutes from a DEAE column and its susceptibility to hyaluronidase digestion. This study supports earlier findings showing increased rates of macromolecular synthesis in osteoarthritis, and in addition, an even greater synthetic rate for hyaluronic acid is demonstrated. |